In biochemistry why sucrose does not react with benedicts solution until treated with acid?
i did a test in food. i added sucrose with benedicts solution and put it in hot water for 10 min, i then took it out of the water and cooled it down, it had not changed colour until i treated it with acid and it became an orange colour after another 10 min in the water
Answers:
Sucrose is a double sugar (disaccharide) of glucose and fructose. Glucose is a reducing sugar (reacts with Benedict's, Fehling's, and Tollen's solutions), because of the oxidation of it's aldehyde (-CH=O) group. Fructose is a reducing sugar because of the oxidation of its keto (>C=O) group. In sucrose, the glucose is bound through its aldehyde group to the keto group of fructose. Therefore, sucrose is a nonreducing sugar, because the two groups are masked. Treatment with acid hydrolyzes sucrose to glucose and fructose, and the sugar mixture reduces Benedict's solution.
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Answers:
Sucrose is a double sugar (disaccharide) of glucose and fructose. Glucose is a reducing sugar (reacts with Benedict's, Fehling's, and Tollen's solutions), because of the oxidation of it's aldehyde (-CH=O) group. Fructose is a reducing sugar because of the oxidation of its keto (>C=O) group. In sucrose, the glucose is bound through its aldehyde group to the keto group of fructose. Therefore, sucrose is a nonreducing sugar, because the two groups are masked. Treatment with acid hydrolyzes sucrose to glucose and fructose, and the sugar mixture reduces Benedict's solution.
this question was asked and resolved recently.you should really look for answers already published before posing the same question again!
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