Do invitrogen restriction enzymes need BSA?
Can't find any info in the catalogue, was just wondering if the enzyme would perform better with BSA. Has anyone compared a digest with and without?
Answers:
Depends on the enzyme, but in general it doesn't hurt.
A long time ago BSA was always used before they found out that some enzymes work equally well without it.
I am not aware of any case that BSA inhibited the reaction (at least at the usual concentration), so I always add it.
BSA stabilises enzymes, most of the invitrogen restriction enzymes work fine without it however it will help some of them especially during longer reactions. It can't hurt to add it to your reaction especially if you are having problems with the activity.
The restriction endonuclease storage buffer contains BSA to help stabilize the enzymes. Further addition of BSA in the reaction cocktail helps in maintaining efficiency. However most of the commom enzymes work just as well without it.
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Answers:
Depends on the enzyme, but in general it doesn't hurt.
A long time ago BSA was always used before they found out that some enzymes work equally well without it.
I am not aware of any case that BSA inhibited the reaction (at least at the usual concentration), so I always add it.
BSA stabilises enzymes, most of the invitrogen restriction enzymes work fine without it however it will help some of them especially during longer reactions. It can't hurt to add it to your reaction especially if you are having problems with the activity.
The restriction endonuclease storage buffer contains BSA to help stabilize the enzymes. Further addition of BSA in the reaction cocktail helps in maintaining efficiency. However most of the commom enzymes work just as well without it.
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