Answer me just in simple words. What is the use of making standard curves in real time PCR?
What is is the objective of of Standard curve in RT-PCR
Answers:
RT-PCR can be used to wok out the amount of mRNA of a particular gene in a sample. To know how much mRNA you have, you need to make a standard curve using samples of a known concentration. Once you've got the curve, You can use the graph to work out how much you have in your sample.
To know how a particular gene changes over time, such as during germination, or in response to changes in environmental conditions.
Real-time polymerase chain reaction has been used to detect changes in gene expression in a tissue in response to an administered pharmacological agent and is thus an important technique in drug discovery and testing.
Primarily used in quantitative RT-PCR, a standard curve allows a scientist to determine how much target RNA is in an unknown sample by comparing the intensity of amplification to points on the standard curve.
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Answers:
RT-PCR can be used to wok out the amount of mRNA of a particular gene in a sample. To know how much mRNA you have, you need to make a standard curve using samples of a known concentration. Once you've got the curve, You can use the graph to work out how much you have in your sample.
To know how a particular gene changes over time, such as during germination, or in response to changes in environmental conditions.
Real-time polymerase chain reaction has been used to detect changes in gene expression in a tissue in response to an administered pharmacological agent and is thus an important technique in drug discovery and testing.
Primarily used in quantitative RT-PCR, a standard curve allows a scientist to determine how much target RNA is in an unknown sample by comparing the intensity of amplification to points on the standard curve.
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